Sample collection for trace element can be used after that.Ī non-additive tube (plain tube) can be drawn before coagulation studies, especially when using a butterfly needle, to allow for dead space in the tubing. Thus the order of draw follows the broad principles of: filling up the “clean” blood culture tubes first, followed by filling of coagulation tubes (for accurate analysis of the coagulation time), then non-additive serum tube or serum separator tube, followed by lithium/sodium heparin, then EDTA tubes, and then sodium fluoride tube. Sodium EDTA contamination resulting in low calcium and high sodium). Cross-contamination of tube additives resulting in spurious results (e.g.Inadvertent contamination of the sample through handling- (In other words, if you are taking a blood culture, if you stick your needle in a dirty EDTA tube before you fill up the culture tubes, you may pick up the bacteria in the EDTA tube and get a contaminated sample).The order of draw is important because the act of filling itself can sometimes affect the blood sample through: If only a coagulation specimen is being drawn, draw 1-2 mL into another blue top (3.2 sodium citrate) tube, discard, and then collect the specimen. A trained phlebotomist using the correct order of draw ensures they obtain a quality sample to be used for diagnostic purposes to provide accurate results. Each tube is differentiated by the tube additive and color. Order of draw refers to the sequence with which one fills the blood tubes, either through the needle holder or through the syringe. Order of draw is the tube sequence a certified phlebotomist needs to follow while collecting blood. It is important to fill the blood tubes in a fixed order of draw (whether you are using the vacutainer system or syringe) to reduce the risk of tube additive contamination.
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